Materials:
-Inoculating loop -25 grams of nutrient agar powder -Bunsen Burner -K 12 E.Coli Broth -18 petri dishes -Water -5 Empty Clean Bottles -Kettle -Microwave -Raw Ginger -Turmeric Powder -Salt -Medium Sized Cheesecloth -Tweezers -Scissors -50 Coffee filters -Ruler -Pencil -Goggles -Gloves -Small Teapot -Funnel |
Method:
Preparation
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4. Afterwards when all 3 dishes were done we placed them all in the incubator upside down so the condensation from the lid would not drip onto the agar. We let them sit inside the incubator for 48 hours.
5. Next we made our 5 solutions for testing. The first solution we made was ginger. We peeled the ginger and then grated it. We took 2 tablespoons of ginger and pushed it through a cheesecloth collecting the juice into a small container. The juice of the ginger, the ginger, and 2 tablespoons of water were added to a small bottle and were then mixed. The same thing was done for garlic. For the turmeric and salt 2 tablespoons were added to 2 tablespoons of water and they were then mixed inside their own bottles. Lastly we made the control liquid by boiling water and allowing it to cool and then pouring it into a bottle
6. Lastly we drew a circle on one of the coffee filters with a diameter of 2 cm. We then cut out the circle and made 50 more like it. (We only needed 45 but we made 5 extra just in case).
Experiment
7. To start we set the incubator to 37 degrees.
8. One hour before the experiment we took out the 15 petri dishes and let them warm up to room temperature, as they were in the fridge for a considerably long period of time. Before we began the experiment we wore gloves for safety. Once the plates were at room temperature we took an inoculating loop and we flamed it then we placed it in one of the petri dish with the e coli and we picked up a small amount that covered the tip of the loop and we then streaked it along the surface. Then we rotated the plate 90 degrees as we and proceeded to pick up the same amount of E.Coli and streaked in the same formation -refer to diagram below-. Afterward we closed the lid and we picked up a piece of the coffee filter circles with one of the tweezers and we dipped it inside one of the remedies, and we let it sit or a few seconds. Then we took it out and held it to remove any excess, and we carefully placed it inside the petri dish. We put 3 circles in each petri dish. We did this for all 5 remedies. Once they were each done we placed them inside the incubator which was already set to 37 degrees.
(the white circles represent the coffee filter paper circles which have been dipped inside the remedy)
5. Next we made our 5 solutions for testing. The first solution we made was ginger. We peeled the ginger and then grated it. We took 2 tablespoons of ginger and pushed it through a cheesecloth collecting the juice into a small container. The juice of the ginger, the ginger, and 2 tablespoons of water were added to a small bottle and were then mixed. The same thing was done for garlic. For the turmeric and salt 2 tablespoons were added to 2 tablespoons of water and they were then mixed inside their own bottles. Lastly we made the control liquid by boiling water and allowing it to cool and then pouring it into a bottle
6. Lastly we drew a circle on one of the coffee filters with a diameter of 2 cm. We then cut out the circle and made 50 more like it. (We only needed 45 but we made 5 extra just in case).
Experiment
7. To start we set the incubator to 37 degrees.
8. One hour before the experiment we took out the 15 petri dishes and let them warm up to room temperature, as they were in the fridge for a considerably long period of time. Before we began the experiment we wore gloves for safety. Once the plates were at room temperature we took an inoculating loop and we flamed it then we placed it in one of the petri dish with the e coli and we picked up a small amount that covered the tip of the loop and we then streaked it along the surface. Then we rotated the plate 90 degrees as we and proceeded to pick up the same amount of E.Coli and streaked in the same formation -refer to diagram below-. Afterward we closed the lid and we picked up a piece of the coffee filter circles with one of the tweezers and we dipped it inside one of the remedies, and we let it sit or a few seconds. Then we took it out and held it to remove any excess, and we carefully placed it inside the petri dish. We put 3 circles in each petri dish. We did this for all 5 remedies. Once they were each done we placed them inside the incubator which was already set to 37 degrees.
(the white circles represent the coffee filter paper circles which have been dipped inside the remedy)
9. We allowed them to sit inside the incubator for 24 hours and once they were done we took them out carefully keeping them upside down, so any water would not drip. We then went and examined the zones of inhibition with a ruler and we recorded the area of each circle which included the initial coffee filter itself and any surrounding area that lacked bacterial growth. We used the diameter to find out the area of the circle. Once we recorded the data we were done the experiment.